look at the attachments. I need someone who knows how to use PyMol.
i need it in 24 hour.PyMOL – Introduction
Proteins play essential roles in living organisms. Their functions are not only restricted to catalytic (e.g., Enzymes) but also structural (e.g., keratin and collagen). Essential to protein function is its structure, and thus, it is necessary to be able to visualize the structure of a protein. Several attempts have been made to obtain the structure of several proteins, and over the past several years, several proteins have been characterized and deposited into the Protein Data Bank (PBD).
The files on the PBD are visualized using software tools that enable the data to be read as interactive pictures. Visualization tools help in the viewing of the 3D structure of the protein, communicate and draw some conclusions on protein interactions both for binding (such as inhibitors.) and catalytic (e.g., substrates). The visualization software includes SWISS PBD (DeepView), Pymol, and Chimera. In this lab, we will be focusing on PyMol and learn some basics.
PyMOL – Activity 1 – Lysozyme
Lysozyme is a model enzyme in protein crystallography: is relatively robust, easy to purify, and crystallizes in many crystal forms in a wide range of conditions.
In 1965, lysozyme became the first enzyme, and only second protein, to have its structure solved. Prior to structure solution, lysozyme was known to have the ability to destroy bacterial cell walls- the body’s own natural antibiotic. However, the mechanism by which lysozyme actually carried out this reaction was less clear and was finally elucidated upon examination of the lysozyme crystal structure.
Today, crystallographers regularly use lysozyme for calibration of X-ray instruments, and professors use it in the lab to train new generations of scientists in structural biology and chemistry. Because of its superior crystallization propensity, lysozyme has even been fused to hard-to-crystallize proteins and enabled the solution of those structures.
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